Curricula

Hemepath Fellow: Molecular/Cytogenetic Education for Hematopathology Fellows

Basic Molecular Knowledge

Cytogenetic Assays

- Karyotype

- FISH

- Microarray

Molecular Assays

Specimen Considerations

PCR Overview

Specialized PCR Techniques

- Allele-specific PCR

- Reverse transcriptase PCR (RT-PCR)

- Quantitative PCR (qPCR)

- Digital droplet PCR (ddPCR)

Sanger Sequencing

Next Generation Sequencing

- Target enrichment

- Library preparation

- Variant calling

- Interpretation

Molecular/Cytogenetics Testing in Myeloid Neoplasms

Molecular Methods Used to Evaluate Myeloid Neoplasia

Chronic Myeloid Neoplasms and Precursor States

- Clonal Hematopoiesis and Clonal Cytopenias

- Myelodysplastic Syndromes

- Myeloproliferative Neoplasms

- Myelodysplastic/Myeloproliferative Neoplasms

- Acute Myeloid Leukemia

- Mastocytosis

- Myeloid Neoplasms with Germline Predisposition

Molecular/Cytogenetics Testing in Lymphoid Neoplasms

Overview of Molecular/Cytogenetic Methods Used to Evaluate Lymphoid Neoplasia

Mature Lymphoid Neoplasms: Clonality Testing

Mature Lymphoid Neoplasms: Recurrent Cytogenetic Abnormalities

Mature Lymphoid Neoplasms: Specialized Molecular Assays

Immature Lymphoid Neoplasms

Molecular/Cytogenetics Testing in Rare Hematopoietic Neoplasms

"Myeloid/Lymphoid Neoplasms With Eosinophilia and Rearrangement of PDGFRA, PDGFRB, or FGFR1, or With PCM1-JAK2"

Mixed Phenotype Acute Leukemia

Histiocytic/Dendritic Cell Neoplasms

Molecular/Cytogenetic Testing in Benign Hematology

Bone Marrow Failure Syndromes

MGP Fellow Practice Alignment: Alignment of Fellowship Training with Practice Patterns for Molecular Pathologists

Molecular genetic principles

- Concepts

- Nucleic acid chemistry

Molecular techniques/methods

- Amplification

- Southern blot analysis

- Gel fragment analysis

- Multi-analyte platforms

- Nucleic acid purification

- Proteomics

- Gene editing

PCR technologies

- Allele specific

- ddPCR

- Fluorescent

- Methylation specific

- MLPA

- Qualitative

- Quantitative

- RT-PCR

- Primer/probe design

Sequencing technologies

- CNV

- Fusion

- Pyrosequencing

- RNA sequencing

- NGS panel (somatic/cancer)

- NGS panel (germline/inherited disease)

- Whole exome sequencing

- - Constitutional

- - Somatic

- Whole genome sequencing

- - Constitutional

- - Somatic

Sanger sequencing

Single-gene assays

Solid tumor mutational profiling

Microsatellite instability

Microarrays

- Gene expression

- SNP constitutional

- SNP somatic

- Array CGH

- Other arrays

Fluorescence in situ hybridization

- FISH prenatal

- FISH heme

- FISH solid tumors

Cytogenetics (karyotyping)

- Cancer

- Constitutional

Genetic counseling

- Bayesian analysis

- Ethical/legal issues

- Familial cancer clinic

- Genetics clinic

- Perinatal clinic

- Consent

Pharmacogenomics

- Cancer

- Psychiatric

- Coagulation

- HLA based

- Methods

Identity testing and HLA

- HLA

- Forensic

- Parentage

- Bone marrow engraftment

Molecular microbiology

- Molecular detection (nonviral)

- Viral detection-qualitative

- Viral load quantitation

- Molecular antimicrobial resistance testing

- Microbial sequencing

- Microbiome

- Public health

Assay verification

Assay validation

- ROI business justification

- Assay planning

- Bioinformatics and IT

- Reporting and nomenclature

- SOP document writing

- Wet bench work

QA/QI

- Assay troubleshooting

- IQCP development

- QC signoff

- QA report/data generation

- Clinical reporting

- Validation/verification

Laboratory management

- Budget/billing

- CAP inspection

- Coding and reimbursement

- Ethics

- Laboratory operations

- Personnel hiring and management

- Proficiency testing submission/review

- Regulatory issues

- Root cause analysis

Professional development

- Job search skills

- Presentation/attendance at meetings

- Role modeling/practice exposure/shadowing

- Tumor board participation

- Writing

- Wellness and resilience

Research

- Projects/idea development

- IRB submission

- Grant writing/securing funding

- Research result reporting: external

- Research result reporting: internal

Informatics

- Databases

- LIS

- Ordering/reporting

- Workflow development

- Workflow quality control

Bioinformatics

- Technical aspects

- "Variant calling, annotation, interpretation"

- - Germline

- - Somatic